wnt3 plasmid  (Addgene inc)

Journal: BMC cancer

Article Title: WNT3 promotes chemoresistance to 5-Fluorouracil in oral squamous cell carcinoma via activating the canonical β-catenin pathway.

doi: 10.1186/s12885-024-12318-2

Figure Lengend Snippet: Fig. 3 Downregulation of WNT3 enhances 5FU efficacy in 5FU-resistant OSCC cells. A, B RT-qPCR and western blot evaluations of WNT3 expression post-knockdown in 5FU-R cells. C, D Decreased IC50 of 5FU in 5FU-R cells after WNT3 knockdown. E, F Inhibited proliferation under 5FU exposure in 5FU-R cells after transfection with siWNT3. G and K Reduced colony-forming potential under 5FU exposure in 5FU-R cells after transfection with siWNT3. H and L Increased apoptosis under 5FU exposure in 5FU-R cells following WNT3 knockdown. I and M Decreased wound-healing (100×) under 5FU exposure in 5FU-R cells after WNT3 knockdown. J and N Diminished migration and invasion (100×) under 5FU exposure in 5FU-R cells after WNT3 knockdown

Article Snippet: 293T cells at 80% confluence were co-transfected with the WNT3 plasmid or its control vector with Top-flash (Addgene, USA) and Renilla plasmids (Promega, USA) using the Lipofectamine 2000.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Knockdown, Transfection, Migration

Journal: Journal of Assisted Reproduction and Genetics

Article Title: LncRNA-NEAT1 blocks the Wnt/β-catenin signaling pathway by targeting miR-217 to inhibit trophoblast cell migration and invasion

doi: 10.1007/s10815-024-03124-7

Figure Lengend Snippet: The results of dual luciferase reporter gene analysis. ( A ) The expression of relative luciferase activity, and effect of miR-217 on luciferase reporter gene activity of NEAT1 mRNA 3'UTR. ( B ) The expression of relative luciferase activity, and effect of miR-217 on luciferase reporter gene activity of Wnt3 mRNA 3'UTR. T-Test was used for data analysis. Data were presented as mean ± SEM. * p ≤ 0.05 vs. Mimics NC group

Article Snippet: Moreover, the constructed Wnt3-Mut or Wnt3-WT luciferase reporter (Yesen Biotechnology, China) plasmid was co-transfected into HTR8/SVneo cells with mimic-NC or miR-217-5p mimic.

Techniques: Luciferase, Expressing, Activity Assay

Journal: eLife

Article Title: The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer

doi: 10.7554/eLife.77376

Figure Lengend Snippet: AGS cells were transfected with Wnt3-mCherry and LifeAct. After 24 hr, the AGS cells were imaged in a 35 mm dishes with a Leica TCS Leica SP8 confocal microscope, using 63×objective. This video lasts 70 s, containing 8 frames.

Article Snippet: The following plasmids were used in transfections and/or microinjections: pCS2 + membrane-mCherry , pCAG-mGFP membrane-bound GFP (Addgene 14757), pEGFP-N1 Flot2-GFP , pEGFP-N1 ΔN-Flot2-GFP , 7×TRE SuperTOPFlash-NLS-mCherry , JNK KTR-mCherry , pCS2 + LifeAct GFP, pCS2 + Rab5 GFP (Jim Smith Group), Rab7-eGFP (from Rüdiger Rudolf), pEGFP-N1 LAMP1-mTurq2 (Addgene #98828), IRSp534K-mCherry/GFP , pCS2 + Wnt8a-mCherry , pCS2 + Ror2-mCherry (cloned in with ClaI and XbaI), pEGFP-N3 mRor2-∆CRD-GFP (XhoI-XhoI mRor2 insert taken from pcDNA-mRor2, subcloned into SalI site of pEGFP-N3 vector), pCS2 + Ror2-eBFP2 (cloned by inserting eBFP2 into pCS2 + Ror2 plasmid using XbaI and SnaBI), pCS2 + Wnt3-mCherry (cloned from Addgene plasmid pcDNA3.2-Wnt3 (#35909) into pCS2±mCherry vector using ClaI and XbaI).

Techniques: